Arnhem, 11 March 2013 – Transplantation of mesenchymal stem cells cultivated on the surface of nanofibrous meshes could be a novel therapeutic strategy against post-prostatectomy erectile dysfunction (ED), conclude the authors of a study which is to be presented at the 28th Annual EAU Congress later this week.
The study was conducted by a group of Korean scientists and will be awarded 3rd prize for best abstract in non-oncology research on the opening day of the congress.
During their investigation, the group aimed to examine the differentiation of human mesenchymal stem cells cultivated on the surface of nanofibrous meshes (nano-hMSCs) into neuron-like cells and repair of erectile dysfunction using their transplantation around the injured cavernous nerve (CN) of rats.
“The objectives of the study reflect a very pertinent need in today’s urology practice,” said the lead author of the investigation Prof. Y.S. Song of Soonchunhyang University School of Medicine in South Korea. “Post-prostatectomy erectile dysfunction results from injury to the cavernous nerve that provides the autonomic input to erectile tissue. It is a common complication after radical prostatectomy which decreases the patient’s quality of life”.
“Although advances in equipment and surgical techniques reduce this complication, patients still experience erectile dysfunction after radical prostatectomy,” he explained.
Treatment of phosphodiesterase 5 inhibitors shows insufficient effectiveness in the treatment of post-prostatectomy ED and it is believed that the transplantation of stem cells cultivated on the surface of nanofibrous meshes can promote cavernous neuronal regeneration and repair erectile dysfunction.
In the course of the study, the synthesised polymer was electrospun in a rotating drum to prepare nanofibrous meshes and hMSCs were prepared and confirmed. Eight week old male Sprague-Dawley rats were divided into 4 groups of 10 each, including sham operation (group 1), CN injury (group 2), hMSCs treatment after CN injury (group 3) and nano-hMSCs treatment after CN injury (group 4). Immediately after the CN injury in group 4, nano-hMSCs encircled the injured CN. Erectile response was assessed by CN stimulation at 2, 4 weeks. Thereafter, penile tissue samples were harvested and examined using morphological analysis and immuno-histochemical stain against nerves (nestin, tubulin βIII and map2), endothelium (CD31,vWF) and smooth muscle (smooth muscle actin).
The results of the study revealed that at 2, 4 weeks, transplantation of nano-hMSCs increased the expression levels of cavernous neuronal, endothelial and smooth muscle makers more than hMSCs alone.
Additionally, nano-hMSCs increased the neuronal differentiation of mesenchymal stem cells more than hMSCs alone. At 2, 4 weeks, the mean percent collagen area of caversnosum increased following CN injury and recovered after transplantation of nano-hMSCs more than hMSCs alone.
At 2, 4 weeks, the group with CN injury had significantly lower erectile function than the group without CN injury (p<0.05). The group transplanted with hMSCs showed higher erectile function than the sham operation group (p<0.05), whereas the group transplanted with nano-hMSCs showed higher erectile function than the group with hMSCs alone (p<0.05).
The authors of the study concluded that nano-hMSCs differentiated into neuron-like cells and their transplantation repair erectile dysfunction in the rats with CN injury. These findings have high potential for the development of follow-up research projects.
“The outcomes of the current study could be a starting point for investigating clinical application of autologous adipocyte derived mesenchymal stem cells cultivated on the nonofiber to the injured caverneous nerve after radical prostatectomy,” said Prof. Song.
“This is necessary to evaluate the effectiveness and safety of transplantated human mesenchymal stem cells cultivated on the surface of nanofibrous meshes against post-prostatectomy erectile dysfunction in patients with cavernous nerve injury.”
Source of this news : http://www.eurekalert.org/pub_releases/2013-03/eaou-sct031113.php
Medical science and its innovations has always helped the human race in being healthy and living a long life. There have been many diseases which were considered to be fatal in the past years but now they are curable. People are no more afraid of those diseases. For example small pox and tuberculosis are two of those diseases which have been responsible for finishing lives of millions of people. However today they are easily curable with allopathic medicine. Similarly many other newly discovered diseases which are not curable by allopathic medication can be cured by stem cells therapy.
Stem cell treatment is a latest cell biology invention or method which has received a lot of attention of biologist, researchers across the globe. This treatment is the only best alternative to traditional or current methods of treating diseases. In this therapy stem cells are harvested from patient’s body only. Then after processing those cells are injected to the damaged part of the diseased person’s body for the purpose of healing disease or damage. These are basically immature cells which can be differentiated in to other types of the cells and then can develop in to new cells or organs.
In many tissues they serve as a sort of internal repair system, dividing essentially without limit to replenish other cells as long as the person or animal is still alive. When one divides, each new cell has the potential either to remain as is or become another type of cell with a more specialized function, such as a muscle cell, a red blood cell, or a brain cell. Stem cell treatment is less cost effective way of treatment as compared to all other ways. We all know that people suffering from cancer undergo painful and expensive chemotherapy. But as far as stem cell treatment is concerned, patients get treated without any painful surgery or procedure.
Stem cell information is very significant to us in order to dig deeper and fathom the issues associating stem therapy. Human body is made up of cells that function to different parts of the body. Cells are microscopic matters, their performance inside is a big factor on how our body in general performs. They are like human too; they have life stages, life span and susceptible to infections. Cells act like a driver and human is the car; when they are sick they might bump the car and as a human we will set sick as well.
Stem cell treatment is the newly discovered kind of treatment known to be more effective than the other treatments available today. Other treatments do not ensure cure form the diseases, however stem cell treatment ensures cure form disease. It is due to the fact that new cells are formed which are healthy and replace the cells causing disease in the person. Success rate of this treatment is enormous. People go for the treatment in lethargic condition and come back on their feet with a healthy smile on their faces.
At stemRx we have developed and following the Treatment Protocol for Osteoarthritis. It is briefly explained below.
Mesenchymal stem cells, or MSCs, are multipotent stromal cells that can differentiate into a variety of cell types,including: osteoblasts (bone cells), chondrocytes (cartilage cells), and adipocytes (fat cells).
Mesenchymal stem cells (MSCs) are adult autologous stem cells found in the bone marrow. However, mesenchymal stem cells can also be isolated from other tissues including cord blood, peripheral blood, fallopian tube, and fetal liver and lung. Mesenchymal stem cells are a distinct entity to the mesenchyme, embryonic connective tissue which is derived from the mesoderm and differentiates to form hematopoietic stem cells.Because of their multi-potent capabilities, mesenchymal stem cell (MSC) lineages have been used successfully in animal models to regenerate articular cartilage and in human models to regenerate bone.
Current treatment for cartilage damage in osteoarthritis focuses on surgical interventions such as arthroscopic debridement, microfracture, and cartilage grafting/transplant, total joint replacement. These procedures have complications than hoped, are invasive, and often entail a prolonged recovery time. MSCs can be harvested from the iliac crest through Bone Marrow Aspiration, expanded using the patient’s own growth factors from platelet lysate, then successfully implanted to increase cartilage volume in an adult human knee.
We present a review highlighting the developments in cellular and regenerative medicine in the arena mesenchymal stem cell therapy by successful harvest, expansion, and transplant of autologous mesenchymal stem cells into an adult human knee that repairs the cartilage, ligaments & meniscal damage & lubricate the joint space.
For More information on Osteoarthritis treatment using stem cells visit http://www.stemrx.in